Compound name
BMS-986176
Protein family
Protein Kinase
Target name
AAK1
Affinity biochemical (nM)
2
Affinity on-target cellular (nM)
2
CG-Set
Kinase set
Recommended Concentration
100 nM
Compound EUbOPEN ID
EUB0002902a
SMILES
CC(C)C[C@@](N)(C)COC1=C(C(F)F)N=C(C2=CC(C(F)F)=NC=C2)C=C1
InChIKey
RKAHOQATMSONTM-IBGZPJMESA-N
NCBI gene ID
22848
UniProt ID
Q2M2I8
Synonyms
KIAA1048, DKFZp686K16132
Mode of action
Inhibitor
Affinity biochemical definition
IC50
Affinity biochemical assay type
Caliper assay (using 22 µM ATP)
Affinity Biochemical Source Knowledge
https://pubmed.ncbi.nlm.nih.gov/35257579/
Affinity biochemical relation
=
Affinity on-target cellular definition
IC50
Affinity on-target cellular assay type
Western Blot assay (inhibition of AAK1 phosphorylation using HEK293 cells)
Affinity on-target cellular source knowledge
https://pubmed.ncbi.nlm.nih.gov/35257579/
Affinity on-target cellular relation
=
Selectivity platform
Kinase panel (literature)
Selectivity platform number of targets
396
Selectivity remarks
Screened at 1 µM, closest targets in the screen: BIKE, CRIK, MNK1, MNK2, % of inhibition not given in paper, https://pubmed.ncbi.nlm.nih.gov/35257579/;
Screened against panel of 249 kinases (literature), in-vitro potency of closest targets:
In-vitro potency (enzymatic assay): IC50(BIKE) = 1900 nM, https://pubmed.ncbi.nlm.nih.gov/35257579/;
In-vitro potency (enzymatic assay): IC50(GAK) = 800 nM, https://pubmed.ncbi.nlm.nih.gov/35257579/;
In-vitro potency (enzymatic assay): IC50(CRIK) = 410 nM, https://pubmed.ncbi.nlm.nih.gov/35257579/;
In-vitro potency (enzymatic assay): IC50(LATS2) = 660 nM, https://pubmed.ncbi.nlm.nih.gov/35257579/;
In-vitro potency (enzymatic assay): IC50(MKN1) = 160 nM, https://pubmed.ncbi.nlm.nih.gov/35257579/;
Screened at CEREB-like safety panel (literature) against 41 targets, in-vitro potency of closest target: IC50(NET) = 390 nM, all other targets >6 µM https://pubmed.ncbi.nlm.nih.gov/35257579/;
Screened against panel of 249 kinases (literature), in-vitro potency of closest targets:
In-vitro potency (enzymatic assay): IC50(BIKE) = 1900 nM, https://pubmed.ncbi.nlm.nih.gov/35257579/;
In-vitro potency (enzymatic assay): IC50(GAK) = 800 nM, https://pubmed.ncbi.nlm.nih.gov/35257579/;
In-vitro potency (enzymatic assay): IC50(CRIK) = 410 nM, https://pubmed.ncbi.nlm.nih.gov/35257579/;
In-vitro potency (enzymatic assay): IC50(LATS2) = 660 nM, https://pubmed.ncbi.nlm.nih.gov/35257579/;
In-vitro potency (enzymatic assay): IC50(MKN1) = 160 nM, https://pubmed.ncbi.nlm.nih.gov/35257579/;
Screened at CEREB-like safety panel (literature) against 41 targets, in-vitro potency of closest target: IC50(NET) = 390 nM, all other targets >6 µM https://pubmed.ncbi.nlm.nih.gov/35257579/;
Selectivity Source Knowledge
https://pubmed.ncbi.nlm.nih.gov/35257579/
Selectivity Number of Off-targets
4