Chemical probes are cell-active, selective, highly validated research tools that can be used to decipher the biology of their target. They are useful assets for example, in phenotypic assays, or as a starting point for medicinal chemistry campaigns. Chemical probes developed by the EUbOPEN consortium are peer reviewed by an external committee and available upon request. The probes are released with a structurally similar inactive negative control compound (where feasible) and fulfill the following criteria:
| Potency | ≤ 100 nM in a biochemical or biophysical assay |
| Selectivity |
≥ 30-fold over related proteins |
| Evidence of target engagement in cells | ≤ 1 µM for the druggable target or 10 µM for shallow PPI targets |
| Cytotoxicity | ≥ 10 µM, unless target mediated |
For more information, or to request a probe, click on the probe link below.