Chemical probes are cell-active, selective, highly validated research tools that can be used to decipher the biology of their target. They are useful assets for example, in phenotypic assays, or as a starting point for medicinal chemistry campaigns. Chemical probes developed by the EUbOPEN consortium are peer reviewed by an external committee and available upon request. The probes are released with a structurally similar inactive negative control compound (where feasible) and fulfill the following criteria:
| Potency | ≤ 100 nM in a biochemical or biophysical assay |
| Selectivity |
≥ 30-fold over related proteins |
| Evidence of target engagement in cells | ≤ 1 µM for the druggable target or 10 µM for shallow PPI targets |
| Cytotoxicity | ≥ 10 µM, unless target mediated |
For more information, or to request a probe, click on the probe link below.
| Probe name | Target | Negative control | Web link | Probe package |
|---|---|---|---|---|
| JA310 | MST3 (Mammalian STE20-like protein kinase 3)/ STK24 (Serine/threonine-protein kinase 24) MST4 (Mammalian STE20-like protein kinase 4)/ STK26 (Serine/threonine-protein kinase 26) | JA262 | JA310: A Chemical Probe for MST3/4 | EUbOPEN_ProbeSheet_JA310_v1.pdf208.28 KB |
| BI-3812 (inhibitor) | BCL6 (BCL6 transcription repressor, Zinc Finger Protein 51) | BI-5273 | BI-3812: A Chemical Probe for BCL6 | EUbOPEN_ProbeSheet_BI_3812_v1.pdf236.36 KB |
| BI-3802 (degrader) | BCL6 (BCL6 transcription repressor, Zinc Finger Protein 51) | BI-5273 | BI-3802: A Chemical Probe for BCL6 | EUbOPEN_ProbeSheet_BI_3802_v1.pdf234.29 KB |
| JP3000 | RXRA, RXAB, RXRG | JP3001 | JP3000: A Chemical Probe for RXR | EUbOPEN_ProbeSheet_JP3000_v1.pdf179.46 KB |
| S213726 | Kelch-like ECH-associated protein 1, KEAP1 (KLHL19) | S214489 | EUbOPEN_ProbeSheet_S213726_v1.pdf219.41 KB | |
| SGC-GSK3-1 | GSK3A, GSK3B | SGC-CDKL5/GSK3-1N | SGC-GSK3-1: A Chemical Probe for GSK3⍺ and GSK3β | EUbOPEN_ProbeSheet_GSK3_v1.pdf262.03 KB |
| SGC-CDKL5/GSK3-1 | STK9, GSK3A, GSK3B | SGC-CDKL5/GSK3-1N | SGC-CDKL5/GSK3-1: A Chemical Probe for CDKL5, GSK3⍺, and GSK3β | EUbOPEN_ProbeSheet_CDKL5GSK3_v1.pdf288.71 KB |
| SGC-CAF382-1 | STK9, PITALRE, PCTAIRE, PCTK1, PCTAIRE2, PCTK2, PCTAIRE3, PCTK3 | SGC-CAF268-1N | SGC-CAF382-1: A Chemical Probe for CDKL5, CDK9, CDK16, CDK17, and CDK18 | EUbOPEN_ProbeSheet_CAF382_v1.pdf293.65 KB |
| BAY-7081 | PDE9A (Phosphodiesterase 9A) | BAY-7424 | BAY-7081: A Chemical Probe for PDE9A | EUbOPEN_ProbeSheet_BAY_7081_v1.pdf149.05 KB |
| JNJ-4355 | MCL1 apoptosis regulator, BCL2 family member (BCL2L3) | JNJ-78732576 | JNJ-4355: A Chemical Probe for MCL1 | EUbOPEN_ProbeSheet_JNJ_4355_v1.pdf180.68 KB |
| BI-3231 | HSD17B13 (Hydroxysteroid 17-beta dehydrogenase 13, SCDR9) | BI-0955 | BI-3231: A Chemical Probe for HSD17B13 | EUbOPEN_ProbeSheet_BI_3231_v1.pdf181.21 KB |
| JNJ-42226314 | MGLL (Monoglyceride lipase) | JNJ-8034 | JNJ-42226314: A Chemical Probe for MGLL | EUbOPEN_ProbeSheet_JNJ_42226314_v1.pdf156.97 KB |
| TP-060 | GCLC (Glutamate-Cysteine Ligase Catalytic Subunit), GCS | TP-060n | TP-060: A Chemical Probe for GCLC | EUbOPEN_ProbeSheet_TP_060_v2.pdf171.74 KB |
| LIJTF500025 | LIMK1 / LIMK2; LIM domain kinase 1/2 | LIJTF500120 | LIJTF500025: A Chemical Probe for LIMK1/2 | EUbOPEN_ProbeSheet_LIMK1_2_v1.pdf160.13 KB |
| JA397 | CDK14 / CDK15 / CDK16 / CDK17 / CDK18; Cyclin-dependent kinase 14/15/16/17/18 | JA314 | JA397: A panTAIRE Chemical Probe | EUbOPEN_ProbeSheet_panTAIRE_v1.pdf159.62 KB |
| SGC-PI5P4Kγ/MYLK4-1 | PI5P4Kγ and MYLK4 | SGC-PI5P4Kγ/MYLK4-1N | SGC-PI5P4Kγ/MYLK4-1: A Chemical Probe for PI5P4Kγ and MYLK4 | EUbOPEN_ProbeSheet_PI5P4Kgamma_v1.pdf193.67 KB |
| SGC-CK2-2 | CK2, CSNK2 | SGC-CK2-2N | SGC-CK2-2: A Chemical Probe for CK2 | EUbOPEN_ProbeSheet_CK2_2_v1.pdf226.18 KB |
| BAY-805 | USP21 (ubiquitin-specific-processing protease 21) | BAY-728 | BAY-805: A chemical probe for USP21 | EUbOPEN_ProbeSheet_BAY-805_v1.pdf174.79 KB |
| FHT-2344 | SMARCA2/ SMARCA4 (SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2/4) | FHT-5908 | FHT-2344: A Chemical Probe for SMARCA2/4 | EUbOPEN_ProbeSheet_FHT_2344_v1.pdf188.78 KB |
| BI-7190 | BPTF (Bromodomain PHD Finger Transcription Factor) | BI-4827 | BI-7190: A Chemical Probe for BPTF | EUbOPEN_ProbeSheet_BI-7190_v1-1.pdf205.49 KB |